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Genetics Course Page Fall 2019

Minot State University

Genetics/ Biol 215

Course Page

Fall 2019

 

Announcements:
Grades submitted!  See recent email.  Best Wishes for your winter break.
Point total (updated December 23) = 466 points
Use this grade calculator to double check your percentage.
 
Guidelines for final:  Here 
 
Morning Lab Gel 1
 
Morning Lab Gel 2
 
Afternoon Lab Gel 1
 
Afternoon Lab Gel 2
 
 
Exam 4 will NOT be Friday, Dec. 13th.  I have decided to incorporate material taught since exam 3 into the final exam.  
 
 
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Lectures

Lecture 1

Lecture 2

Lecture 3     CRISPR and Sickle Cell Anemia

Lecture 4     More on Cell cycle control

Lecture 5

Lecture 6

Lecture 7.     Paper --Down syndrome.         Tumor suppressor  in DSCR

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Lecture 8

Lecture 9

Lecture 10

Lecture 11--INDEPENDENT STUDY FOR 9-27-19

Lecture 12

Lecture 13

Lecture 14

Lecture 15

Lecture 16

Lecture 17

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Lecture 18

Lecture 19

Lecture 20

Lecture 21

Lecture 22

Lecture 23

Lecture 24

Lecture 25

Lecture 26

Lecture 27

Novembern 8th, 2019---We will just finigh the concepts of lecture 27, go over lab quiz 2 and look at transformation plates.  

Lab 8--Observations and discussion.

Lecture 28

Lecture 29

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Lecture 30

Lecture 31--Few new slides from last lecture

Lecture 32

Lecture 33

Lecture 34

Lecture 35

Lecture 36

Lecture 37--You are responsible for all content of this lecture.

 

Lab.   F2 mapping data here

Lab Manual--2019

lab report guidelines

Lab 1--preparation of onion root

Lab 1--Handouts for Sept. 5th, 2019

Example lab report  Do not plagiarize. This is not a perfect report.   *This example report was written before students participated in the growing of their own roots.  Don't forget that part of the protocol. 

Print both below for Lab 2

Lab 2---Meiosis Part 1

Lab 2---Meiosis Part 2

MEIOSIS POWERPOINT CLICK HERE 

Lab 3--Mendel Part 1

Lab 4--Analyisis of corn data    Compiled Data here (2 different sheets to spreadsheet).  2-page pdf hard copy of data: HERE

Guidelines for lab 3-4

Lab 5--Introduction to Drosophila and X-linkage. Introductory lecture

Lab 6--Human traits (We will also set up Drosophila crosses today)

Lab 7---Analyisis of human traits---Population genetics.  Hardy-Weinberg. Class data here:

Lab report 5/7 guidelines

Lab 8---Bacterial Transformation--DNA is the genetic material. Lab 8--Observations and discussion.

Lab 9---Finish up bacterial transformation, discuss GFP and basics of gene regulation AND analyze Drosophila F1 and introduce 3-point mapping.-- New lab materials provided in lab.   Bring this:Observations and discussion.

Presentation on 3 point mapping

Presentation on gene regulation (GFP)

Lab 10--Polymerase Chain Reaction--determining your PV92 genotype/molecular genotyping   

PCR LECTURE

PCR  (Animation)

 Lab 11 ---analysis of PCR products.--Analyzing our PV92 genotype.  DNA gel electrophoresis.

 

It is essential that you are prepared for this lab.  Read your lab handout from Lab 10 to refresh your memory of PCR and to begin to understand the dimorphic nature of the PV92 locus.  PV92 has 2 alleles. Predict  your genotype.  Review animation on electrophoresis. 

 

Electrophoresis animation: http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

 

100 bp ladder

1 kb ladder

 

 

Lecture Documents/Assigned Reading

Syllabus  Fall 2019

lecture summary page

Check out this advance in the treatment of Tay-Sachs disease. 
 

 

Another recessive human genetic disease with huge advance in treatment--Check out new drug development for Cystic Fibrosis

 

Exam Prep

Topics lists

Exam 1 topics list

Exam 2 topics list

Exam 3 topics list

Guidelines for final exam: here

 

Practice Problems

Problem Set 1    Key/Solutions

Problem Set 2.    Key/Solutions

Pedigree problems--including X-linkage  Hemophilia story--questions

Problem Set 3     Key/solutions

Problem Set 4--linkage/recombination mapping.   Key/Solutions

 

Animations

Mitosis

Meiosis

Replication 1

Replication 2

Transcription (prokaryotes) and more Transcription (eukaryotes)

Protein Synthesis Animation--translation

animations---translation

 

Below this line---Ignore!  Old material

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Drosophila F2 data analysis--guidelines

 

 

PV92 gels here.  9 am lab     1 pm lab

Paper 3 Dog gentetics paper

Paper 4--Variation in size in dogs--read abstract.

Paper 5---X-inactivation molecular mechanism

Lab 7---take home lab on Polygenic/Continuous traits

Lab 8---Drosophila part II and Introduction to recombination mapping Part I and Part II.

 

 Drosophila Part III 

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

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AttachmentSize
TRANSFORMATION LABdisscussion2013.doc33.5 KB
National Geo dog genetics story.pdf3.66 MB
pointer lab 1.docx147.79 KB
lecture37.ppt1.18 MB
guidelinesforfinal-1.doc25.5 KB
Lecture 26-2018 Fall.ppt2.34 MB
Lecture 27-2018 Fall.ppt1.76 MB
Lecture 30-2018 Fall.ppt2.91 MB
Drosophila part III.doc29 KB
Lecture 37-2018 Fall.ppt3.12 MB
Lecture 37 2019.pptx1.87 MB
guidelinesforfinal-2019.doc31.5 KB