Molecular Biology Course Page--Spring 2019 (H. Super)

Lab 2--- pGLO plasmid purification. (Be sure to bring your analysis handout from Lab 1 and have hade detailed observations of your 6 plates. We will start with discussion.

Lab 3---Basic analyis of pGLO plasmid. Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--electrophoresis of pGLO plasmid restriction fragments---making a restriction map. Agarose gel prep. Here

Electrophresis animation http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

Gel pictures pGLO-gel 1 pGLO gel-2

Lab 5--Bioinformatics using pGLO sequence. PDFpGLO sequence file. Word pGLO sequence file. If you want to bring your own computer, please do. I will have department laptops for your use.

Lab 6--Conventional/endpoint PCR--amplifying GFP sequence using your primers

Lab 6- Part 2/Analyzing endpoint PCR

Lab 7--qPCR (real time PCR).

Lab 8--Purifying GFP protein

Lab 9 --SDS-PAGE. GFP separation using polyacrylamide gel electrophoresis. AMENDED PROTOCOL

Previous year MATERIAL BELOW THIS LINE--not current for 2019

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Lab 8---Real-time/quantitative PCR

Lab 8--Part 2 (print, read, and bring to lab)

View these docuemnts before lab. Powerpoint with Ct curves qPCR data Standard curves

Lab 6--- pGLO sequence file.

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis

Discussion

Data 2

ANIMATIONS

More terrific animations here!

The Meselson-Stahl Experiment

Transcription

Lab 7--SDS-PAGE

Lab 9 real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

Molecules of the cell--Reading

GFP readings

lactoferrin gfp pGLO lactoferrin

walnut GFP.jpg walnut GFP

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous year's class. Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

ANIMATIONS

The Meselson-Stahl Experiment

Animation Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos