Molecular Biology Course Page--Spring 2019 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Spring 2019








Syllabus 2019

google book 




Powerpoint lectures:

Lecture 1

Lecture 2

Lecture 3   Decoding Watson---Documentary

Lecture 4

Lecture 5

Lecture 6




Lab syllabus

Lab 1Bacterial Transformation/DNA IS the genetic material   Transformation animation here. Transformation analysis handout: here

Lab 2--- pGLO plasmid purification.   (Be sure to bring your analysis handout from Lab 1 and have hade detailed observations of your 6 plates.  We will start with discussion.



Previous year  MATERIAL BELOW THIS LINE--not current for 2019




Topics Lists for Exams









   Transformation discussion handout: here  Great video about GFP: here

Lab 2--Purification of pGLO plasmid

Lab 3--Determining concentration of plasmid DNA and quality of PGLO plasmid sample.  Using restriction enzymes.  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--pGLO restriction mapping/Electrophoresis and Beyond!







Lab 5--Bioinformatics---Preparing for PCR with pGLO.  PDFpGLO sequence file.  Word pGLO sequence file If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 6--Purifying GFP from E. coli transformants

Lab 7---endpoint PCR.  Usinging PCR primers of your design!

Lab 7 part 2---DNA gel electrophoresis---analyzing the PCR amplicon

Lab 8---Real-time/quantitative PCR

Lab 8--Part 2  (print, read, and bring to lab)

View these docuemnts before lab.  Powerpoint with Ct curves  qPCR data  Standard curves



Check out these awesome resourses:  

Science Daily (news site)

DNA Learning Center


IFL science :)







Lab 3--Analysis of the pGLO plasmid  

Lab 4--DNA electrophoresis of pGLO plasmid.  Analyzing electrophoresis and contructing a restriction map.


Lab 5---purifying GFP from E. coli transformants.

Lab 6--- pGLO sequence file.  If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 7---Conventional PCR/Endpoint PCR

Lab 7---DNA agarose gel to analyze PCR

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis


Data 2







More terrific animations here!

The Meselson-Stahl Experiment







Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)




Good website for GFP info:











Lecture handouts week of 2-22-10 molecular documents week 3-22-10


Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP




Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR








The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation link here--check out protein folding! Scientific Video Site

Telomerase demos


Horn Lab - Projects



lecuture summary page

Biol 480 2012lecture3.ppt2.3 MB
Biol 480 2012lecture4.ppt3.95 MB
1.9 MB
Biol 480 2019 Lecture 4.pptx6.98 MB