Molecular Biology Course Page--Spring 2019 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Spring 2019




Check out these awesome resourses:  

Science Daily (news site)

DNA Learning Center

IFL science :)



Syllabus 2019

google book 


Powerpoint lectures:

Lecture 1

Lecture 2

Lecture 3   Decoding Watson---Documentary

Lecture 4

Lecture 5

Lecture 6  Youtube video on DNA Topology

Lecture 7

Lecture 8

Lecture 9   Epigenetics chapter notes


Lecture 10--updated

Lecture 11

Lecture 12

Lecture 13  Histones/replication paper

Lecture 14

Lecture 15

Lecture 16

Lecture 17

Lecture 18


Lecture 19

Lecture 20

Lecture 21

Lecture 22

Lecture 24

Lecture 25


Lecture 26

Lecture 27

Lecture 28

Lecture 29-part 1

Lecture 29-part 2

Lecture 30

Lecture 31

Lecture 32

Lecture 33

Lecture 34-1   Insulin translation regulation (Read introduction and study figure 8)      

 alpha and beta globin translation regulation  (read first 2.5 pages)

Lecture 34-2


Topics Lists for Exams

Topics exam 1

Topics exam 2

Topics exam 3

Topics exam 4




Lab syllabus

Lab 1Bacterial Transformation/DNA IS the genetic material   Transformation animation here. Transformation analysis handout: here

Lab 2--- pGLO plasmid purification.   (Be sure to bring your analysis handout from Lab 1 and have hade detailed observations of your 6 plates.  We will start with discussion.

Lab 3---Basic analyis of pGLO plasmid.  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--electrophoresis of pGLO plasmid restriction fragments---making a restriction map. Agarose gel prep. Here

Electrophresis animation

Gel pictures pGLO-gel 1           pGLO gel-2

Lab 5--Bioinformatics using pGLO sequence.   PDFpGLO sequence file.  Word pGLO sequence file. If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 6--Conventional/endpoint PCR--amplifying GFP sequence using your primers

Lab 6- Part 2/Analyzing endpoint PCR

Lab 7--qPCR (real time PCR).

Lab 8--Purifying GFP protein

Lab 9 --SDS-PAGE. GFP separation using polyacrylamide gel electrophoresis.  AMENDED PROTOCOL




Previous year  MATERIAL BELOW THIS LINE--not current for 2019





Lab 8---Real-time/quantitative PCR

Lab 8--Part 2  (print, read, and bring to lab)

View these docuemnts before lab.  Powerpoint with Ct curves  qPCR data  Standard curves

Lab 6--- pGLO sequence file.  

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis


Data 2







More terrific animations here!

The Meselson-Stahl Experiment







Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)




Good website for GFP info:











Lecture handouts week of 2-22-10 molecular documents week 3-22-10


Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP




Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR








The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation link here--check out protein folding! Scientific Video Site

Telomerase demos


Horn Lab - Projects



lecuture summary page

molec. topics list 4-2014.docx88.54 KB
Lab8GFPpurification.doc48.5 KB
Lecture 30.pptx5.12 MB